Why is my guitar out of tune when I fret?
If your frets are worn, notes played on those frets will be out of tune. Sometimes frets pop up a bit throwing notes out of pitch at those frets. If you're noticing notes out of tune throughout the neck it might be time to either have the frets dressed (leveled) or after excessive use, replaced.
Can truss rod affect tuning?
Tightening your truss rod means it creates extra pull away from the strings. This means it will pull your strings out-of-tune. To avoid any problems, loosen your strings before you make adjustments to your truss rod. If there is a back bow or you're experiencing buzzing frets, it means you need to loosen the truss rod.