Topmusicanswers
What is FRET technique?
The technique of fluorescence resonance energy transfer (more commonly referred to by the acronym FRET), when applied to optical microscopy, permits determination of the approach between two molecules within several nanometers (see Figure 1), a distance sufficiently close for molecular interactions to occur.
What determines FRET efficiency?
The FRET efficiency can be calculated by subtracting the donor intensity in the presence of the acceptor from its intensity after photobleaching the acceptor, and then normalizing this value to the donor intensity after bleaching.
What is FRET imaging?
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1–10 nm range.
What is FRET in PCR?
FRET is an acronym for Fluorescence Resonance Energy Transfer. In this type of detection system, two flanking primers are needed; in addition, two oligonucleotide probes are also required (as compared to only one in the Taqman format). The probes must be located internal of the. flanking.
